(1967) A method for the hybridization of nucleic acid molecules at low temperature. (1977) Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Application to specific modification of DNA. (1981) Photoreaction of thymidine with primary amines. Saito, I., Sugiyama, H., Furukawa, N., Matsuura, T.Streptococcus mutans, Operon and Biofilms ResearchGate, the professional. (1988) Rapid, reversible staining of northern blots prior to hybridization. We show here that inactivation of pdhD, putatively encoding a subunit of the pyruvate dehydrogenase complex, impairs. (1980) Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. (1994) One-hour downward capillary blotting of RNA at neutral pH. (1973) Gel electrophoresis of RNA under denaturing conditions. Reijnders, L., Sloof, P., Sival, J., Borst, P.(1977) Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Shewry 1 show more details PDF Full text Abstract Soon after the introduction of the Southern blotting procedure (1), an analogous technique, known as northern blotting, was developed for the analysis of RNA sequences (2). (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Northern Analysis and Nucleic Acid Probes Authors: Paolo A. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. A Laboratory Guide for Isolation and Characterization. In this protocol, we describe how northern blot analysis is used to identify gene expression patterns at the RNA level in human cancer cells as well as in cancerous and normal tissues. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. (1989) Molecular Cloning: A Laboratory Manual. (1994) Isolation and characterization of Ribonucleoprotein complexes, in (Higgins, S. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Northern blotting and 5-rapid amplification of. There are several advantages to Northern blotting analysis. (1979) Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Adjacent downstream genes walK, EF1195, EF1196, and EF1197 were co-transcribed and detect antisense walR RNA. This chapter details the method for analyzing RNA by Northern blotting, which basically involves the isolation of RNA, its size fractionation by electrophoresis, transfer to membrane, and detection by nucleic acid hybridization and autoradiography. (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Furthermore, by demonstrating that antibodies against PSMβ peptides inhibited bacterial spread from indwelling medical devices, we have provided proof of principle that interfering with biofilm detachment mechanisms may prevent dissemination of biofilm-associated infection. Our study establishes in vivo significance of biofilm detachment mechanisms for the systemic spread of biofilm-associated infection and identifies the effectors of biofilm maturation and detachment in a premier biofilm-forming pathogen. epidermidis biofilm structuring and detachment in vitro and dissemination from colonized catheters in a mouse model of device-related infection. epidermidis peptides - the β subclass of phenol-soluble modulins (PSMs) - promote S. Here we have demonstrated that specific secreted, surfactant-like S. However, the molecular determinants of biofilm dissemination are unknown. Infections involving biofilms frequently develop on indwelling medical devices in hospitalized patients, and Staphylococcus epidermidis is the leading cause of infection in this setting. Biofilm-associated infections are difficult to eradicate and represent a significant reservoir for disseminating and recurring serious infections. Biofilms are surface-attached agglomerations of microorganisms embedded in an extracellular matrix.
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